Hello all,
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I’m the molecular biology lab manager and assay developer at a lab in Sandy Springs, GA. I’m currently in the process of developing an onychosis PCR panel to detect various nail pathogens, and I would like know the actual pathogen prevalence many of you are observing among the patients you serve. This will help me make a better-informed decision as to what pathogens to include and omit on our PCR panel so that the patients receive medically relevant test results and don’t get billed for unnecessary tests. If interested in helping me with the knowledge you possess, please feel free to answer any of the questions below:
I appreciate any help you're able to give,
- The main categories of nail pathogens we plan to screen for are Fungi (primarily Dermatophytes), Saprophytic Molds, Candida-Species Yeasts, and Pseudomonas aeruginosa. Are there any other pathogen categories that should be considered?
- Out of the Fungi, Mold, and Yeast pathogen categories, what multiple species would it be medically relevant to isolate because it requires a species-specific treatment (e.g. T. rubrum, Epidermophyton, C. albicans, etc.)?
- What are the common treatments prescribed for Pseudomonas aeruginosa, which leads to Green Nail Syndrome?
- What are the common treatments prescribed for Fungi (also treatments for specific species, if applicable, like Terbinafine for Dermatophytes)?
- What are the common treatments prescribed for Yeasts (also treatments for specific species, if applicable)?
- What are the common treatments prescribed for Saprophytic Molds (also treatments for specific species, if applicable)?
- Finally, have you seen a prevalence or need to identify the pathogens Botryodiploida theobromae, Onychochola canadensis, and/or Pyrenochaeta unguis-hominis?
MD
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