PURPOSE: Although the RICE (Rest, Ice, Compression, and Elevation) treatment
has been recognized as the gold standard of first aid treatment for sports injuries, we
and the others previously demonstrated that a transient icing treatment immediately
after skeletal muscle injury impaired muscle regeneration. However, the information
about the influence of icing treatment following skeletal muscle injury remains limited.
This study was, therefore, to investigate the impact of icing as a first-aid treatment on
qualitative recovery of damaged muscle in terms of fibrosis and myosin heavy chain
(MyHC) profile.
METHODS: Male Wistar rats (9-10 weeks of age) were randomly assigned to control
(Con), injured, and injured with icing groups (Ice). Bupivacaine (BPVC) was injected
into slow soleus muscles bilaterally in order to induce muscle injury in the two injury
groups. Application of icing treatment (ice pack, 0°C for 20 min) to the icing group
was carried out immediately after the BPVC injection. At 3 days-4 weeks after BPVC
injection, soleus muscles were removed and analyzed.
RESULTS: Compared with the Con group, a significant increase in fibrotic area
was observed after 2 weeks following injury in the injured groups, but after 1 week
following injury in the Ice group (P < 0.05). This area was also tended to be higher
in Ice than in injured animals during 1-4 weeks of recovery period. In addition, the
number of Tcf4-positive nuclei, a fibroblast marker, located in interstitial spaces in
both injured groups markedly increased 1 week after BPVC injection. The numbers
were tended to be more magnified in the Ice group than in the injured group, then their
number in both injured groups gradually degreased thereafter. Injury-related de novo
appearance of embryonic, neonatal, IId/x, and IIb MyHC isoforms was noted in both
injured groups 1 week after BPVC injection. These MyHC isoforms were significantly
decreased toward the undetectable level thereafter. However, the embryonic MyHC
isoform was still detectable in icing, but not in injured, animals 4 weeks after BPVC
injection.
CONCLUSIONS: Our results suggested that icing treatment following skeletal muscle
injury will have a negative impact on recovery process (fibrosis and normalization of
MyHC profile) of regenerating muscle.
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